Role of Post-Intraoperative Radiation Therapy Wound Fluids in Interaction with White Blood Cells on Cancer Cell Growth Role of PIWF on cancer cell growth

Belal Delshad (1), Hamed Abadijoo (2), Hossein Simaee (3), Mohammad Ali Khayamian (4), Mohammadreza Ghaderinia (5), Seyed Mojtaba Yazdanparast (6), Jalil Beheshti (7), Khosro Shamsi (8), Maryam Avatefi Afkham (9), Sepideh Mansouri (10), Mohammad Esmaeil Akbari (11), Mohammad Abdolahad (12)
(1) Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Iran, Islamic Republic of,
(2) Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran, Iran, Islamic Republic of,
(3) Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran, Iran, Islamic Republic of,
(4) Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran, Iran, Islamic Republic of,
(5) Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran, Iran, Islamic Republic of,
(6) Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran, Iran, Islamic Republic of,
(7) Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Iran, Islamic Republic of,
(8) Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Iran, Islamic Republic of,
(9) Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Iran, Islamic Republic of,
(10) Radiation Oncology Research Center (RORC), Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran/ Recombinant Proteins Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran, Iran, Islamic Republic of,
(11) Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Iran, Islamic Republic of,
(12) Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran/ UT&TUMS Cancer Electronics Research Center, Tehran University of Medical Sciences, Tehran, Iran, Iran, Islamic Republic of
https://doi.org/10.32768/abc.20241189-95

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Issue
Vol. 11 No. 1 (2024): Volume 11, Issue 1, February 2024
Submitted
December 16, 2023
Published
January 31, 2024
Keywords:
    breast cancer, tumor microenvironment, Intraoperative, Radiation therapy, Immune System, Cytokines
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Abstract

Background: Intraoperative radiation therapy (IORT), is a promising method which has been widely applied in breast cancer lumpectomy. Although its effect on destructing remaining cancer cells was approved, maintaining or draining post intraoperative radiation therapy wound fluids (PIWF) is challenging. Moreover, the roles of immune cells in interaction with PIWFs have not been studied before which is the main investigation of this paper.
Methods: Surgical wound fluids were collected from 24 IDC patients one day after lumpectomy. The patients were divided into control and IORT groups. The collected wound fluids were centrifuged for 20 minutes at 2000 rpm. The concentration of tumor-associated
cytokines and inflammasomes were recorded using the immunoassays.
Results: PIWFs stimulate the residue of cancer cells in cavity sides causing disease progression. Here we have focused on the effect of PIWFs on the proactivation or deactivation of WBCs in the tumor bed environment. By sequential imaging in time-transient
intervals from the interaction between WBCs and cancer cells, PIWFs have no additive proactivating effect on immune cells.
Conclusion: PIWFs have significant roles in proliferation of cancer cells but did not show an observable role in pro-activating immune cells against cancer cells. The functions of immune cells did not show any independent proactivation in the presence of PIWFs with
respect to their activation in the presence of blood serum. It seems that draining the PIWFs may be required. In future research, we will use tumor samples of the patients instead of cell lines to better investigate the personalized immune-tumor interactions of patients.

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References

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Authors

Belal Delshad
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Hamed Abadijoo
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran
Hossein Simaee
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Mohammad Ali Khayamian
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran
Mohammadreza Ghaderinia
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran
Seyed Mojtaba Yazdanparast
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran
Jalil Beheshti
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Khosro Shamsi
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Maryam Avatefi Afkham
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Sepideh Mansouri
Radiation Oncology Research Center (RORC), Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran/ Recombinant Proteins Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Mohammad Esmaeil Akbari
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
profmeakbari@gmail.com (Primary Contact)
Mohammad Abdolahad
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/ Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran/Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran/ UT&TUMS Cancer Electronics Research Center, Tehran University of Medical Sciences, Tehran, Iran
1.
Delshad B, Abadijoo H, Simaee H, Khayamian MA, Ghaderinia M, Yazdanparast SM, Beheshti J, Shamsi K, Avatefi Afkham M, Mansouri S, Akbari ME, Abdolahad M. Role of Post-Intraoperative Radiation Therapy Wound Fluids in Interaction with White Blood Cells on Cancer Cell Growth: Role of PIWF on cancer cell growth. Arch Breast Cancer [Internet]. 2024 Jan. 31 [cited 2024 Jun. 17];11(1):89-95. Available from: https://www.archbreastcancer.com/index.php/abc/article/view/863
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