Regulation of IL-10 by Herbal and Hormonal Therapy: The Impact of Eleutherine Bulbosa and Tamoxifen in an Experimental Breast Cancer Model Effects of Eleutherine bulbosa–Tamoxifen on interleukin-10
Abstract
Background: Breast cancer is the most common cancer affecting women worldwide, with the number of cases and deaths continuing to rise. This study focuses on evaluating the single and combined effects of Eleutherine bulbosa ethanol extract and tamoxifen in reducing interleukin-10 levels in BALB/c mice with breast cancer models.
Methods: This study used a laboratory experimental approach with a post-test-only control group design, using 36 female Mus musculus BALB/c strain mice (aged 8-10 weeks) and randomly divided into six groups: negative and positive control groups. The intervention groups were given Eleutherine bulbosa ethanol extract at a dose of 180 mg/kg BW every 14 days, tamoxifen was given at a dose of 10 mg/kg BW every 2 days for 7 days within a 14-day period, a combination of Eleutherine bulbosa ethanol extract and tamoxifen, and tamoxifen and Eleutherine bulbosa ethanol extract. Effectiveness was evaluated based on interleukin-10 levels in serum.
Results: The study found that the combination of Eleutherine bulbosa ethanol extract and tamoxifen significantly reduced Interleukin-10 levels in BALB/c mice with breast cancer modeling. The extract Eleutherine bulbosa ethanol extract reduced Interleukin-10 by 31.4%, while tamoxifen reduced it by 59.1%. The strongest synergistic effect was observed when combined with tamoxifen, reducing Interleukin-10 levels by 64.6%, indicating its effectiveness in suppressing the immunosuppressive response supporting tumor growth.
Conclusion: Tamoxifen and Eleutherine bulbosa extract showed synergistic effects in reducing IL-10 levels, suggesting enhanced immunomodulatory action compared to monotherapy. This technique could enhance breast cancer treatment efficacy by diminishing immunosuppression.
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