An in Vitro Model Study on the Effectiveness of Electrical Pulse Mediated Herbal Therapy on Breast Cancer Effectiveness of electrical pulse mediated herbal therapy

Gowri Sree V (1), Poompavai S (2)
(1) Division of High Voltage Engineering, Department of Electrical and Electronics Engineering, College of Engineering, Anna University, Chennai, India, India,
(2) Division of High Voltage Engineering, Department of Electrical and Electronics Engineering, College of Engineering, Anna University, Chennai, India, India


Background: Breast cancer is the most life-threatening cancer in women all over the world. Considering the detrimental side effects of standard chemo drugs, plant-based drugs can be used effectively. This work aims to investigate the effect of herbal extracts mediated by electrical pulses on breast cancer cell lines.
Methods: Three different samples were chosen (Turmeric, Pepper, Neem), shade dried, and extracted using the soxhlet method, with ethanol as a solvent. These extracts were used as an anticancer drug against three breast cancer cell lines (MCF-10A, MCF-7, MDA-MB-231). Further, BTX ECM830 electroporator was used to generate electrical pulses of different parameters to enhance the uptake of extracts into the cancer cells. Realtime MT Assay was used to obtain the viability of breast cancer cell lines. A comparative study was conducted to assess the effect of each treatment on different breast cancer cell lines.
Results: Treatments were more susceptive to cancerous cell lines than normal cells. Low intensity, high duration electrical pulses with herbal extracts showed a higher cytotoxic effect than other treatments. A notable increase in cell death was observed in combination treatments compared to single treatments. The lowest viability of 4% was obtained for synergetic treatment of electrical pulses and herbal extract.
Conclusion: In the current study, we found for the first time that herbal extracts in combination with electrical pulses exhibited strong anticancer activity against breast cancer cell lines. This promising treatment can be used efficiently to treat breast cancer without any side effects.

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Breast Cancer Statistics and Resources, 2020. Available from:

Weaver JC. Electroporation of cells and tissues, IEEE Trans. Plasma Sci., vol. 28, no. 1, (2000) 24–33.

Lambert L, Keyomarsi K , Cell cycle deregulation in breast cancer; insurmountable chemoresistance or Achilles heel, in: Breast Cancer Chemosensitivity , Yu D and Hung M-C (eds), New York: Springer Science Business Media (2007) 52–69. doi: 10.1007/978-0-387-74039-3_4.

Gonzalez-Augulo AM, Morales-Vasquez F, Hortobagyi GN. Overview of resistance to systemic therapy in patients with breast cancer, in: Breast Cancer Chemosensitivity , Yu D and Hung M-C (eds), New York: Springer Science Business Media (2007) 1–22. doi:10.1007/978-0-387-74039-3_1.

Poompavai S, Gowri Sree V. Dielectric property measurement of breast—tumor phantom model under pulsed electric field treatment. IEEE Trans. Radiat. Plasma Med. Sci. Vol. 2. (2018) 608–617. doi: 10.1109/TRPMS.2018.2868818

Poompavai S, Gowri Sree V, Kaviya Priyaa A. Electrothermal Analysis of the Breast-Tumor Model during Electroporation. IEEE Transactions on Radiation and Plasma Medical Sciences. 4 (2020) 512-524. doi:10.1109/TRPMS.2020.2967558.

Gehl J, Geertsen PF. Efficient palliation of haemorrhaging malignant melanoma skin metastases by electrochemotherapy, Melanoma Res. 10 (2000) 1–5. doi: 10.1097/00008390-200012000-00011.

Mittal L, Raman V, Camarillo IG, Sundararajan R. Ultra-microsecond pulsed curcumin for effective treatment of triple negative breast cancers. Biochem Biophys Res Communications, 491, 4, (2017) 1015-1020. doi:10.1016/j.bbrc.2017.08.002.

Leontiadou H, Mark AE, Marrink SJ. Molecular dynamics simulations of hydrophilic pores in lipid bilayers’, Biophysical J, 86(4) (2004) 2156–64. doi: 10.1016/j.bbrc.2017.08.002.

Sundararajan R. Electroporation-Based Therapies for Cancer: From Basics to Clinical Applications. Elsevier. New York. USA 2014.

Davey C.L., Kell D.B. The Dielectric Properties of Cells and Tissues: What can They Tell Us about the Mechanisms of Field/Cell Interactions?. In: O’Connor M.E., Bentall R.H.C., Monahan J.C. (eds) Emerging Electromagnetic Medicine. (1990) Springer, New York, NY. doi: 10.1007/978-1-4612-3386-2_2.

Greenwell M, Rahman PK. Medicinal Plants: Their Use in Anticancer Treatment. Int J Pharm Sci Res. 6(10) (2015) 4103-4112. doi: 10.13040/IJPSR.0975-8232.6(10).4103-12.

Sofowora A, Ogunbodede E, Onayade A. The role and place of medicinal plants in the strategies for disease prevention. African journal of traditional, complementary, and alternative medicines: AJTCAM 10(5) (2013) 210–229. doi: 10.4314/ajtcam.v10i5.2.

Yuan H, Ma Q, Ye L, Piao G. The Traditional Medicine and Modern Medicine from Natural Products. Molecules (Basel, Switzerland) 21(5) (2016) 559. doi: 10.3390/molecules21050559.

Prasad S, Aggarwal BB. Chapter 13 Turmeric, the Golden Spice, From Traditional Medicine to Modern Medicine. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. Benie FF, Wachtel-Galor S, Boca Raton (FL): CRC Press/Taylor & Francis; 2011.

Pandey MM, Rastogi S, Rawat AK. Indian traditional ayurvedic system of medicine and nutritional supplementation. Evidence-Based Complementary and Alternative Medicine. 2013;2013. doi: 10.1155/2013/376327

Poompavai S, Gowri Sree V. Anti-Proliferative Efficiency of Pulsed Electric Field Treated Curcuma Longa. (Turmeric) Extracts on Breast cancer cell lines. IETE Journal of Research, 2020, 1799873. doi: 10.1080/03772063.2020.1799873.

Moga MA, Bălan A, Anastasiu CV, Dimienescu OG, Neculoiu CD, Gavriș C. An Overview on the Anticancer Activity of Azadirachta indica (Neem) in Gynecological Cancers. International journal of molecular sciences (2018), 19(12), 3898. doi: 10.3390/ijms19123898

Liu Y, Yadev VR, Aggarwal BB, Nair MG. Inhibitory Effects of Black Pepper (Piper nigrum) Extracts and Compounds on Human Tumor Cell Proliferation, Cyclooxygenase Enzymes, Lipid Peroxidation and Nuclear Transcription Factor-kappa-B, Natural Product Communications, 5 8 (2010) 1253 – 1257. doi: Not Available

Kaefer CM, Milner JA. Herbs and Spices in Cancer Prevention and Treatment. In: Benzie IFF, Wachtel-Galor S, editors. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. Boca Raton (FL): CRC Press/Taylor & Francis, Chapter 17. 2011. Available from:

Poompavai S, Gowri Sree V, Sundararajan R. Enhanced extraction of bioactive compounds from natural herbs by pulsed electric field method. Proceedings of 2019 Annual Meeting of the Electrostatics of America. Rochester. NY. June 2019. doi: Not Available

Holliday DL, Speirs V. Choosing the right cell line for breast cancer research. Breast Cancer Res 13, 215 (2011). doi: 10.1186/bcr2889.

TECHNICAL MANUAL for CellTiter-Glo® 2.0 Assay. Available from:

Montgomery DC. Design and Analysis of Experiments. New York: JohnWiley & Sons, 2013. doi: 10.2174/187152012803833026.

Wang H, Khor TO, Shu L, Su ZY, Fuentes F, Lee JH, Kong AN. Plants vs. cancer: a review on natural phytochemicals in preventing and treating cancers and their druggability. Anti-cancer agents in medicinal chemistry, 12(10) (2012) 1281–1305. doi: 10.2174/187152012803833026.

Cure JC. Cancer an elecrical phenomenon, Resonant, vol.1, no.1, 1991.

Mittal L, Raman V, Camarillo IG, Garner AL, Sundararajan R. Viability and cell cycle studies of metastatic triple negative breast cancer cells using Low voltage electrical pulses and herbal curcumin. Biomed. Phys. Eng. Express, 2018. doi:10.1088/2057-1976/aaf2c3.

Thulasidas J, Gowri Sree V, Lakshya M, Sundararajan R. The Combined Effect of Aqueous Tulasi Extract and Electrical Pulses on the Viability of HeLa Cells. IETE Journal of Research. 2019. doi: 10.1080/03772063.2019.1642804.

Shree TJ, Sree VG, Poompavai S, Sieni E, Sgarbossa P, Camarillo I, et al. Inhibition of proliferation of HeLa cells by pulsed electric field treated Menthapiperita (Mint) extract. IETE. J. Res. (2019) doi: 10.1080/03772063.2019.1626295.

Poompavai S, Gowri Sree V. Synergy of Electrical Pulses and Black Pepper (Piper nigrum) Extracts for Effective Breast Cancer Treatment: An in Vitro Model Study, IETE Journal of Research, (2021). doi: 10.1080/03772063.2021.1896977.

Poompavai S, Gowri Sree V, RamSugitha B. Enhanced Extraction and Improved Anticancer Activity of Neem (Azadirachta indica) Leaves by Electroporation Technique, IETE Journal of Research, (2021) doi: 10.1080/03772063.2021.1962743.

Gowri Sree V, Muthuraman C, Sundararajan R. Electro-cisplatin-therapy for estrogen-receptor positive breast cancers: an in vitro model study. J. Cancer Prev. Curr. Res. Vol. 7, no. 4 (2017) 1-6. doi: 10.15406/jcpcr.2017.07.00245.


Gowri Sree V (Primary Contact)
Poompavai S
V GS, S P. An in Vitro Model Study on the Effectiveness of Electrical Pulse Mediated Herbal Therapy on Breast Cancer: Effectiveness of electrical pulse mediated herbal therapy. Arch Breast Cancer [Internet]. 2022 Mar. 4 [cited 2024 Apr. 21];9(2):162-73. Available from:

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