Breast Cancer Cell Lines, HER2/Neu Phenotype, and a Higher Propensity to Reactive Oxygen Species Production

Zahra Mohammadi Abgarmi (1), Abbas Sahebghadam Lotfi (2), Saeid Abroun (3), Masoud Soleimani (4), Shahla Mohammad Ganji (5), Parvaneh Baktash (6), Arash Moradi (7)
(1) Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran, Iran, Islamic Republic of,
(2) Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran, Iran, Islamic Republic of,
(3) Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran, Iran, Islamic Republic of,
(4) Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran, Iran, Islamic Republic of,
(5) Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran, Iran, Islamic Republic of,
(6) Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran, Iran, Islamic Republic of,
(7) Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran, Iran, Islamic Republic of

Abstract

Background: The reactive oxygen species (ROS) generated in the biological systems play an important role in pathological conditions and specific normal cellular processes, such as signaling pathways and drug sensitivity.
Methods: Intracellular ROS was determined using (2'-7'dichlorofluorescin diacetate) DCFH-DA fluorimetric probe, malondialdehyde as lipid peroxidation index detected by the Thiobarbituric acid reactive substances method. Cells' protein carbonyl contents were assessed with 2,4-Dinitrophenylhydrazine (DNPH) derivatization spectrophotometrically at 360-385 nm.
Results: The oxidative stress induces ROS (p?0.05), MDA (Malondialdehyde) (p?0.05), and protein carbonylation (p?0.01) was significantly higher in HER2-positive BT-474, SK-BR-3, and MDA-MB-453 compared to the HER2-negative MDA-MB-231 and MCF-7 cell lines.
Conclusion: We hypothesized that increased oxidative stress in HER2-positive cell lines is due to the oncogenic function of the HER2 and PI3K/Akt signaling activation, resulting in glycolysis induction. It is assumed that HER2-positive cell lines with high ROS levels are more vulnerable to further damage by increased ROS levels induced by pro-oxidant anticancer agents.

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Authors

Zahra Mohammadi Abgarmi
Abbas Sahebghadam Lotfi
Saeid Abroun
Masoud Soleimani
Shahla Mohammad Ganji
shahlamg@yahoo.com (Primary Contact)
Parvaneh Baktash
Arash Moradi
1.
Mohammadi Abgarmi Z, Sahebghadam Lotfi A, Abroun S, Soleimani M, Mohammad Ganji S, Baktash P, Moradi A. Breast Cancer Cell Lines, HER2/Neu Phenotype, and a Higher Propensity to Reactive Oxygen Species Production. Arch Breast Cancer [Internet]. 2021 Apr. 15 [cited 2024 Nov. 8];:137-42. Available from: https://www.archbreastcancer.com/index.php/abc/article/view/366

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